Q. Biolistics (gene gun) is suitable for
✅ Correct Answer: (A)
introducing rDNA into plant cells
Biotechnology: Principles and Processes MCQs with answers
Dear candidates you will find MCQ questions of 12th Class - Biology | Chapter: Biotechnology: Principles and Processes here. Learn these questions and prepare yourself for coming examinations. You can check the right answer of any question by clicking the option or by clicking view answer button.
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Q. In genetic engineering experiments, restriction enzymes are used for
✅ Correct Answer: (B)
bacterial DNA
Q. The DNA fragments produced by the use of restriction endonucleases can be separated by
✅ Correct Answer: (C)
gel electrophoresis
Q. Plasmids in bacterial cells are
✅ Correct Answer: (B)
extra-chromosomal DNA, which can self-replicate
Q. The DNA polymerase enzyme used in PCR is obtained from
✅ Correct Answer: (D)
Thermus aquaticus
Q. While isolating DNA from bacteria, which of the following enzymes is not used?
✅ Correct Answer: (D)
Lysozyme
Q. Significance of ‘heat shock’ method in bacterial transformation is to facilitate
✅ Correct Answer: (C)
uptake of DNA through transient pores in the bacterial cell wall
Q. Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
✅ Correct Answer: (C)
Extension of primer end on the template DNA
Q. In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be
✅ Correct Answer: (B)
transformants only and not the non-transformants can survive.
Q. The role of DNA ligase in the construction of a recombinant DNA molecule is
✅ Correct Answer: (A)
formation of hydrogen bonds between sticky ends of DNA fragments
Q. ‘Restriction’ in Restriction enzyme refers to
✅ Correct Answer: (B)
prevention of the multiplication of bacteriophage in bacteria
Q. Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
✅ Correct Answer: (D)
Ethidium bromide stained DNA can be seen under exposure to UV light
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