Enzymes are biological catalysts made of proteins that speed up chemical reactions in living organisms without being consumed. Each enzyme acts on a specific substrate and converts it into a product. Enzymes lower the activation energy required for reactions. Their activity depends on temperature, pH, and concentration. The region where the substrate binds is called the active site. Examples include amylase, lipase, pepsin, and DNA polymerase. Enzymes play a vital role in digestion, metabolism, and cellular regulation.