In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is correct?

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Mr. Dubey

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📊 Animal Biotechnology

Q. In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is correct?

(A) Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2
(B) Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2
(C) Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2
(D) CO2 requirement is independent of bicarbonate concentration in the medium

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✅ Correct Answer: (A) Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2

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