Q. Majority of the cereals are highly recalcitrant to Agrobacterium mediated transformation, and so direct transformation methods have been developed to transform such plants. Which of the following direct transformation methods is applicable to intact plant tissues?

• (A) Calcium chloride and PEG-mediated transformation
• (B) Liposome-mediated transformation
• (C) Electroporation
• (D) Transformation using micro projectiles