Q. Phage T7 promoter containing plasmids are used for over-expression of cloned genes because
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Correct Answer: (C)
Exquisite specificity of T7 RNA polymerase to phage promoters
Recombinant DNA Technology MCQs | Page - 1
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Q. Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because
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Correct Answer: (A)
The sequence context at the RB defines the direction of transfer
Q. The restriction endonuclease Eco52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco52I?
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Correct Answer: (B)
XmaIII (C/GGCGG)
Q. Which one of the following statements about Agrobacterium Ti plasmid is correct?
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Correct Answer: (D)
Opine biosynthesis genes are located within the T-DNA segment
Q. A single base pair of DNA weighs 1.1 × 10-21 grams. How many picomoles of a plasmid vector of length 2750 bp are contained in 1 μg of purified DNA?
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Correct Answer: (B)
0.55
Q. Many plasmids have ampr marker. This implies
β
Correct Answer: (C)
The plasmid contains the gene encoding β-lactamase
Q. T4 polynucleotide kinase is used for
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Correct Answer: (B)
Labeling 5' ends of DNA
Q. A gene cannot be isolated from a human genomic DNA library by functional complementation in E. coli because of
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Correct Answer: (B)
The absence of splicing machinery
Q. To isolate a gene coding for glucagon, the cDNA library as to be constructed using mRNA isolated from
β
Correct Answer: (B)
Pancreas
Q. Which one of the following cannot be a recognition sequence for a type II restriction enzyme?
β
Correct Answer: (D)
ATGCCT
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