πŸ“Š Methods and Techniques in Applied Biology
Q. There are two different ways of determining the nucleotide sequence of a nucleic acid: the chemical quencing (Maxam-Gilbert) method and the enzymatic sequencing (Sanger) method. The basic principle/advantage of the Sanger method is
  • (A) The differential interaction of the bases with particular dyes
  • (B) Extension of a synthetic primer and reliable termination of DNA repair synthesis
  • (C) The correlation of restriction sites with the end-label of the DNA
  • (D) The ability to 'sequence' both strands of the DNA duplex simultaneously
πŸ’¬ Discuss
βœ… Correct Answer: (D) The ability to 'sequence' both strands of the DNA duplex simultaneously

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