Q. Which of the following would not possible to address using a Northern Blot?
β
Correct Answer: (A)
Location of restriction sites in a particular gene
Methods and Techniques in Applied Biology MCQs | Page - 1
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Q. In southern blotting . . . . . . . . is separated by gel electrophoresis
β
Correct Answer: (A)
DNA
Q. A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be
β
Correct Answer: (B)
256 bp
Q. Terminal transferase is used
β
Correct Answer: (A)
To add base at the 3' end of the DNA
Q. Mung bean nuclease could be used for
β
Correct Answer: (C)
Trimming single stranded regions in DNA
Q. Many plasmids have Ampicillin marker. This implies
β
Correct Answer: (C)
The plasmid contains the gene encoding β-lactamase
Q. DNA synthesis can be measured by estimating incorporation of radio-labelled
β
Correct Answer: (C)
Thymidine
Q. In agarose gel electrophoresis
β
Correct Answer: (D)
Ethidium bromide can be used to visualize the DNA
Q. A cloning vector consisting of CoS site inserted in a plasmid, used to clone DNA fragments of λ phage is
β
Correct Answer: (B)
Cosmid
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