Q. Which of the following statement is incorrect?

  • (A) Inactivation of a gene for determination of gene function of that gene is called gene knockout
  • (B) Technique used to construct a clone by overlapping fragments of cloned DNA is called chromosome walking
  • (C) A clinical procedure in which a gene or other DNA sequence is used to treat a disease is called gene therapy
  • (D) None of these
πŸ’¬ Discuss
βœ… Correct Answer: (D) None of these

Q. Hybridoma technology is used in

  • (A) Synthesis of haemoglobin
  • (B) Production of alcohol
  • (C) Production of somatic hybrids
  • (D) Synthesis of monoclonal antibodies
πŸ’¬ Discuss
βœ… Correct Answer: (D) Synthesis of monoclonal antibodies

Q. Which statement about restriction enzymes are false?

  • (A) Restriction enzymes cut DNA at specific sequence called recognition sites
  • (B) A restriction enzyme always cut DNA to leave the same sequence at the ends
  • (C) Some restriction enzymes cut the two DNA strands at slightly different points within their recognition site to make a 'sticky' end
  • (D) Restriction enzymes are exonucleases rather than endonucleases
πŸ’¬ Discuss
βœ… Correct Answer: (D) Restriction enzymes are exonucleases rather than endonucleases

Q. Free amino acids can be determined with the help of

  • (A) Heterotrophic bacteria
  • (B) Auxotrophic bacteria
  • (C) Algae
  • (D) Fungi
πŸ’¬ Discuss
βœ… Correct Answer: (B) Auxotrophic bacteria

Q. Reverse genetics means

  • (A) Finding the functions of a ORF
  • (B) Finding the gene responsible for a trait
  • (C) RNA dependent DNA synthesis
  • (D) Converting somatic cell to a germ cell
πŸ’¬ Discuss
βœ… Correct Answer: (B) Finding the gene responsible for a trait

Q. If the linear double stranded genomic DNA of bacteriophage T4 is heated and cooled, one gets both linear and circular molecules because,

  • (A) All linear DNA on heating and cooling attain circular shape
  • (B) The DNA molecules are circularly permuted
  • (C) The DNA molecule has cohesive terminal ends
  • (D) The RNA component of the genomes gets hydrolyzed to results in the circularization
πŸ’¬ Discuss
βœ… Correct Answer: (C) The DNA molecule has cohesive terminal ends

Q. The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?

  • (A) EcoRI (G/AATTC)
  • (B) XmaIII (C/GGCGG)
  • (C) SmaI (CCC/GGG)
  • (D) SacII (CCGC/GG)
πŸ’¬ Discuss
βœ… Correct Answer: (B) XmaIII (C/GGCGG)

Q. The genome sequencing of rice is important because

  • (A) The rice genome is very unique and contains genes not found in other plants
  • (B) The rice genome is very large compared to the DNA of other grains and so more genes will be identified
  • (C) It can identify genes associated with disease resistance, growth capacity, etc
  • (D) It has a rapid life cycle and has many identifiable mutations
πŸ’¬ Discuss
βœ… Correct Answer: (C) It can identify genes associated with disease resistance, growth capacity, etc

Q. Which of the following methods would give you the most precise and accurate information about where and when a given gene is expressed?

  • (A) In situ hybridization
  • (B) DNA microarray
  • (C) Protein microarray
  • (D) Reporter gene fusion including introns
πŸ’¬ Discuss
βœ… Correct Answer: (B) DNA microarray

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