Q. Which of the following statement is incorrect?
✅ Correct Answer: (D)
None of these
Methods and Techniques in Applied Biology MCQs | Page - 3
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Q. The sensitivity of ELISA is
✅ Correct Answer: (C)
10-9 g/ml
Q. Hybridoma technology is used in
✅ Correct Answer: (D)
Synthesis of monoclonal antibodies
Q. Which statement about restriction enzymes are false?
✅ Correct Answer: (D)
Restriction enzymes are exonucleases rather than endonucleases
Q. Free amino acids can be determined with the help of
✅ Correct Answer: (B)
Auxotrophic bacteria
Q. Reverse genetics means
✅ Correct Answer: (B)
Finding the gene responsible for a trait
Q. If the linear double stranded genomic DNA of bacteriophage T4 is heated and cooled, one gets both linear and circular molecules because,
✅ Correct Answer: (C)
The DNA molecule has cohesive terminal ends
Q. The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?
✅ Correct Answer: (B)
XmaIII (C/GGCGG)
Q. The genome sequencing of rice is important because
✅ Correct Answer: (C)
It can identify genes associated with disease resistance, growth capacity, etc
Q. Which of the following methods would give you the most precise and accurate information about where and when a given gene is expressed?
✅ Correct Answer: (B)
DNA microarray
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