Q. The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?

  • (A) EcoRI (G/AATTC)
  • (B) XmaIII (C/GGCGG)
  • (C) SmaI (CCC/GGG)
  • (D) SacII (CCGC/GG)
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βœ… Correct Answer: (B) XmaIII (C/GGCGG)

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