Methods and Techniques in Applied Biology MCQs | Page - 2
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Q. A gene that does not contain introns has an Eco RI site just down stream of the translational termination codon in the 3' untranslated region. The size of the gene is 4 Kbp. The length of 3' UTR is 2 Kbp, the 5' UTR is 1 Kbp and that the open reading frame is 1kbp. Upon probing a southern blot of an Eco RI digested genomic DNA with the radioactively labelled fragment containing only the open reading frame, the size of the band detected by hybridization would be
✅ Correct Answer: (A)
1 Kbp
Q. Cells deficient in hypoxanthine guanine phospho-ribosyl transferase (HGPRT)
✅ Correct Answer: (B)
Synthesis of purine deoxynucleotides by de novo path way
Q. Which of the following statements gives a correct explanation for the use of vectors containing drug resistance genes in the cloning of recombinant DNA (cDNA) molecules?
✅ Correct Answer: (C)
Entry of the vector containing the cDNA and the drug resistance genes into the host cell renders the latter identifiable as it is now resistant to antibiotic drugs
Q. There are two different ways of determining the nucleotide sequence of a nucleic acid: the chemical quencing (Maxam-Gilbert) method and the enzymatic sequencing (Sanger) method. The basic principle/advantage of the Sanger method is
✅ Correct Answer: (D)
The ability to 'sequence' both strands of the DNA duplex simultaneously
Q. The ability to clone an adult animal from the nucleus of a differentiated somatic cell proves that
✅ Correct Answer: (D)
The nuclei of somatic cells contain all the genetic information present in a fertilized egg
Q. For protoplast fusion to be successful in plant cells.
✅ Correct Answer: (C)
DNA between the two cells should be compatible
Q. The process used for separation of protein in polyacrylamide gel is called:
✅ Correct Answer: (C)
Western blotting
Q. The technique which involves addition or deletions of genes is
✅ Correct Answer: (B)
Genetic engineering
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