Q. Which of the following are not valid methods of labelling duplex DNA?
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Correct Answer: (B)
3'-end labelling with polynucleotide kinase
Methods and Techniques in Applied Biology MCQs | Page - 4
Dear candidates you will find MCQ questions of Methods and Techniques in Applied Biology here. Learn these questions and prepare yourself for coming examinations and interviews. You can check the right answer of any question by clicking on any option or by clicking view answer button.
Q. BAC, which can be used to clone large DNA fragments, is derived from
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Correct Answer: (B)
F plasmid
Q. Restriction endonucleases hydrolyzes polynucleotide from
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Correct Answer: (D)
A phosphodiester bond within a specific sequence
Q. ELISA is used to detect viruses where the key reagent is
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Correct Answer: (A)
Alkaline phosphatase
Q. Genetically engineered male sterile crop plants may be produced by inserting
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Correct Answer: (A)
BT toxin gene
Q. Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately
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Correct Answer: (D)
1 : 26
Q. Which of the following is a desirable characteristic for a cloning plasmid?
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Correct Answer: (D)
All of the above
Q. Which of the following could not possibly give rise to a restriction fragment length polymorphism (RFLP)?
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Correct Answer: (D)
An error in RNA splicing that mistakenly removes an exon during RNA processing
Q. Which of the following is a semi-continuous fermentation in which only a small amount of substrate is available at the beginning of the process?
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Correct Answer: (B)
Fed-batch fermentation
Q. Which statement correctly describes sequential steps in cDNA cloning?
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Correct Answer: (A)
Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector
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