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Mr. Dubey • 100.64K Points
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Q. A DNA fragment of 4500 bp has to be tailed with dT residues by using dTTP and the enzyme 'terminal transferase' The stock solution of dTTP that is used as a substrate has concentration of 150 μM. Ten μL of this stock solution is added to a total volume of 200 μL reaction. What will be the concentration of dTTP in the reaction?

  • (A) 7.5 μM
  • (B) 75 μM
  • (C) 0.75 μM
  • (D) 0.075 μM
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