Q. The greatest separation is obtained in which portion of the gel?
  • (A) Lower portion where the anode is
  • (B) Lower portion where the cathode is
  • (C) The separation is uniform all over
  • (D) It varies according to the quantity of the size of the molecules to be separated
πŸ’¬ Discuss
βœ… Correct Answer: (A) Lower portion where the anode is
πŸ“Š Current Advances of Novel Drug Delivery System
Q. If further electrophoresis is used for recovery of DNA from gels, the method is termed as ____________
  • (A) secondary electrophoresis
  • (B) recovery electrophoresis
  • (C) denaturing electrophoresis
  • (D) electro-elution
πŸ’¬ Discuss
βœ… Correct Answer: (D) electro-elution
πŸ“Š Current Advances of Novel Drug Delivery System
Q. If the amount of agarose added is more, the molecular under analysis should have the following characteristic………………..
  • (A) small size
  • (B) large size
  • (C) size has no relation with the amount of agarose
  • (D) the amount of molecules under analysis matters
πŸ’¬ Discuss
βœ… Correct Answer: (A) small size
Q. Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?
  • (A) They are obtained via polymerization between acrylamide and bis-acrylamide
  • (B) The components added for initiating polymerization are ammonium persulphate and TEMED
  • (C) It is casted in horizontal and flat trays
  • (D) TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking
πŸ’¬ Discuss
βœ… Correct Answer: (C) It is casted in horizontal and flat trays
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Q. Gel matrices are generally of two types Agarose and Polyacrylamide. Which of the statements is correct with respect to agarose gels.
  • (A) Agarose is a polysaccharide which is obtained from red algae
  • (B) Agarose is a polysaccharide obtained from fungus
  • (C) It is composed of glucose residues
  • (D) It is obtained via dissolving in its water by boiling in water and then cooling it
πŸ’¬ Discuss
βœ… Correct Answer: (A) Agarose is a polysaccharide which is obtained from red algae
Q. How is the size of molecules under analysis measured?
  • (A) By measuring the distance moved through a ruler
  • (B) By measuring the amount of visualising dye used
  • (C) By running a standard molecule, whose size is known in parallel
  • (D) There is no exact criterion for doing so
πŸ’¬ Discuss
βœ… Correct Answer: (C) By running a standard molecule, whose size is known in parallel
Q. Which one of the following will travel fastest through the gel if the amount of DNA present is same in all?
  • (A) Circular
  • (B) Supercoiled
  • (C) Nicked
  • (D) Supercoiled and circular will move at same speed and faster than nicked
πŸ’¬ Discuss
βœ… Correct Answer: (B) Supercoiled