Q. The physiological mechanism responsible for flowering in response to temperature
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Correct Answer: (B)
Vernalisation
Basic Principles of Biotechnology MCQs | Page - 1
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Q. Which of the following amino acids have side chains that are negatively charged under physiological conditions (i.e., near pH 7)?
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Correct Answer: (A)
Asp
Explanation:
Aspartic acid (Asp) has a carboxyl group in its side chain that loses a proton at physiological pH (~7), making it negatively charged.
Why others are incorrect:
- (B) Histidine (His): Side chain is positively charged or neutral around pH 7 because its imidazole group has a pKa close to physiological pH but usually not negatively charged.
- (C) Tryptophan (Trp): Nonpolar, uncharged side chain.
- (D) Cysteine (Cys): Has a thiol group, which is mostly neutral at physiological pH (can be negatively charged but only under more alkaline conditions).
Final Answer: (A) Asp
Q. Histones have very high percentage of basic amino acids (15 - 30%). For this class of proteins which of the following reagents would be a suitable choice for generating peptides in determination of amino acid sequence of the protein
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Correct Answer: (C)
Trypsin
Q. Number of nucleotide present in FMN is
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Correct Answer: (D)
Zero
Q. If a reaction is at equilibrium, the free energy, ΔG change is
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Correct Answer: (B)
0
Q. Under standard temperature (T) and pressure (P) conditions, 128 g of an ideal gas molecule A occupies a volume of 1 L. The gas molecule A obeys the relationship RT = 0.25 PV. R and V are universal gas constant and ideal gas volume, respectively. The molecule A is
β
Correct Answer: (D)
O2
Q. A peptide has the sequence Glu-His-Trp-Ser- Gly-Leu-Arg-pro-Gly. What is the net charge of the molecule at PH 11.
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Correct Answer: (D)
-2
Q. Which one of the following statements about eukaryotic RNA polymerases I, II and III is false?
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Correct Answer: (D)
The carboxyl end of RNA Pol II contains a short sequence of only seven amino acids which is called the CTD and which may be phosphorylated
Q. Which one of the following is the unit of heat transfer coefficient?
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Correct Answer: (C)
W m-2 K-1
Q. Which one of the following is the most suitable type of impeller for mixing high viscosity (viscosity > 105 cP) fluids?
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Correct Answer: (B)
Helical ribbon
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