πŸ“Š Basic Principles of Biotechnology
Q. Henderson's equation can be respresented as
  • (A) pH=pKa+log [Acid] [Salt]
  • (B) pH=pKa+log [Salt] [Acid]
  • (C) pKa=pH+log [Salt] [Acid]
  • (D) pKa=pH−log [Salt] [Acid]
πŸ’¬ Discuss
βœ… Correct Answer: (B) pH=pKa+log [Salt] [Acid]
πŸ“Š Basic Principles of Biotechnology
Q. The role of calcium in muscle contraction is to
  • (A) Break the cross-bridges as a cofactor in the hydrolysis of ATP
  • (B) Bind with troponin, changing its shape so that the actin filament is exposed
  • (C) Transmit the action potential across the neuromuscular junction
  • (D) Spread the action potential through the T tubules
πŸ’¬ Discuss
βœ… Correct Answer: (B) Bind with troponin, changing its shape so that the actin filament is exposed
πŸ“Š Basic Principles of Biotechnology
Q. Why is the inactivation of a useful technique to determine the function of a gene?
  • (A) Gene inactivation provides information about the expression of a gene
  • (B) Gene inactivation provides information about the cellular location of a gene product
  • (C) Gene inactivation provides an opportunity to identify phenotypic changes associated with the loss of the functional gene
  • (D) Gene inactivation provides information about the structure of the gene product
πŸ’¬ Discuss
βœ… Correct Answer: (C) Gene inactivation provides an opportunity to identify phenotypic changes associated with the loss of the functional gene
πŸ“Š Basic Principles of Biotechnology
Q. Which of the following statements is not true of fatty acids found in most mammalian cells?
  • (A) They usually contain 12 to 20 carbons
  • (B) Polyunsaturated fatty acids contain conjugated double bonds
  • (C) All double bonds are in the cis configuration
  • (D) Free fatty acids are ionized at physiological pH
πŸ’¬ Discuss
βœ… Correct Answer: (B) Polyunsaturated fatty acids contain conjugated double bonds
πŸ“Š Basic Principles of Biotechnology
Q. The isoelectric point of a protein is defined as
  • (A) The pH at which the net charge on the molecule is zero
  • (B) The pH at which all groups are protonated
  • (C) The pH at which all groups are unprotonated
  • (D) The pH at which each acidic group is protonated and each basic group is unprotonated
πŸ’¬ Discuss
βœ… Correct Answer: (A) The pH at which the net charge on the molecule is zero
πŸ“Š Basic Principles of Biotechnology
Q. Prandtl number is the ratio of
  • (A) Thermal diffusivity to momentum diffusivity
  • (B) Mass diffusivity to momentum diffusivity
  • (C) Momentum diffusivity to thermal diffusivity
  • (D) Thermal diffusivity to mass diffusivity
πŸ’¬ Discuss
βœ… Correct Answer: (C) Momentum diffusivity to thermal diffusivity
πŸ“Š Basic Principles of Biotechnology
Q. For a nucleic acid probe to hybridize, which of the following must be true?
  • (A) Both the probe and the sequence to which it hybridizes must be RNA
  • (B) Both the probe and the sequence to which it hybridizes must be DNA
  • (C) The sequence to which it hybridizes must be identical to that of the probe
  • (D) The sequence to which it hybridizes must be complementary to that of the probe
πŸ’¬ Discuss
βœ… Correct Answer: (D) The sequence to which it hybridizes must be complementary to that of the probe
πŸ“Š Basic Principles of Biotechnology
Q. A homogeneous protein of native molecular weight 100,000 gave a single band of molecular weight 50,000 on SDS-PAGE in presence of β-mercaptoethanol. N-terminal analysis gave two amino acids alanine and leucine in equal proportions. Hence the
  • (A) Protein is a homodimer
  • (B) Protein is contaminated with another protein
  • (C) Protein has two polypeptides linked by disulphide bridges
  • (D) None of the above
πŸ’¬ Discuss
βœ… Correct Answer: (A) Protein is a homodimer
πŸ“Š Basic Principles of Biotechnology
Q. In the RNAi regulatory pathway, the DICER enzyme cleaves
  • (A) RNA polymerase into non-functional pieces
  • (B) Single-stranded DNA into repetitive sequence
  • (C) Double-stranded RNA into short strands
  • (D) mRNAs of genes to be repressed
πŸ’¬ Discuss
βœ… Correct Answer: (C) Double-stranded RNA into short strands

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