πŸ“Š Plant Biotechnology
Q. Tobacco leaf discs are transferred with Agrobacterium tumefaciens strain containing binary vector (GUS as reporter gene) with selectable marker neo (kanamycin resistance gene) and then regenerated to plants. The plants are kanamycin resistant, but leaf tissues are negative to GUS assay. The explanation is
  • (A) The plants are transformed for both genes but GUS gene is turned
  • (B) The plants are transformed for only neo gene not the GUS gene
  • (C) The plants are not transformed at all, but the development of kanamycin resistance is due to somaclonal variation
  • (D) All the above
πŸ’¬ Discuss
βœ… Correct Answer: (D) All the above
πŸ“Š Plant Biotechnology
Q. The plant hormone indole-3-acetic acid is derived from
  • (A) Histidine
  • (B) Tyrosine
  • (C) Tryptophan
  • (D) Proline
πŸ’¬ Discuss
βœ… Correct Answer: (C) Tryptophan
πŸ“Š Plant Biotechnology
Q. Hormone pairs required for a callus to differentiate are
  • (A) Auxin and cytokinin
  • (B) Auxin and gibberellin
  • (C) Ethylene and gibberellin
  • (D) Cytokinin and gibberellin
πŸ’¬ Discuss
βœ… Correct Answer: (A) Auxin and cytokinin
πŸ“Š Plant Biotechnology
Q. Resistance to herbicide chlorsulfuron in plants is due to change in
  • (A) Glutamine synthetase
  • (B) Acetolactate synthase
  • (C) Theronine deaminase
  • (D) DNA polymerase
πŸ’¬ Discuss
βœ… Correct Answer: (B) Acetolactate synthase
πŸ“Š Plant Biotechnology
Q. The pungency of mustard seeds is primarily due to secondary metabolites such as isothiocyanate and nitrile. The pungency is usually felt only when the seeds are crushed. This is because of
  • (A) The coat of the intact seeds blocks the pungent volatiles from being released
  • (B) The pungent chemicals are stored as inactive conjugates and compartmentalized from the enzymes that convert them into active chemicals
  • (C) The pungent chemicals are formed only after the reaction with atmospheric oxygen
  • (D) The pungent chemicals are formed only after the reaction with atmospheric carbon dioxide
πŸ’¬ Discuss
βœ… Correct Answer: (B) The pungent chemicals are stored as inactive conjugates and compartmentalized from the enzymes that convert them into active chemicals
πŸ“Š Plant Biotechnology
Q. Majority of the cereals are highly recalcitrant to Agrobacterium mediated transformation, and so direct transformation methods have been developed to transform such plants. Which of the following direct transformation methods is applicable to intact plant tissues?
  • (A) Calcium chloride and PEG-mediated transformation
  • (B) Liposome-mediated transformation
  • (C) Electroporation
  • (D) Transformation using micro projectiles
πŸ’¬ Discuss
βœ… Correct Answer: (B) Liposome-mediated transformation
πŸ“Š Plant Biotechnology
Q. Vincristine and vinblastine, two commercially important secondary metabolites from Catharanthus roseus, are examples of
  • (A) Alkaloids
  • (B) Flavonoids
  • (C) Terpenoids
  • (D) Steroids
πŸ’¬ Discuss
βœ… Correct Answer: (A) Alkaloids
πŸ“Š Plant Biotechnology
Q. Agrobacterium based transformation of protoplasts obtained from dicots is based on the fact that
  • (A) These exhibit strong chromosomal structures
  • (B) These have two cotyledons
  • (C) These exhibits strong wound response
  • (D) These have long tap root system
πŸ’¬ Discuss
βœ… Correct Answer: (C) These exhibits strong wound response
πŸ“Š Plant Biotechnology
Q. Hydrated synthetic seeds which are produced by ion exchange reaction involve mixing the somatic embryos in a solution of
  • (A) Sodium alginate and dropping it in a solution of calcium nitrate
  • (B) Calcium alginate and dropping it in a solution of sodium nitrate
  • (C) Calcium alginate and dropping it in a solution of ammonium nitrate
  • (D) Mannitol and dropping it in a solution of sodium nitrate
πŸ’¬ Discuss
βœ… Correct Answer: (A) Sodium alginate and dropping it in a solution of calcium nitrate
πŸ“Š Plant Biotechnology
Q. Gynogenesis is a process of development of haploid plants
  • (A) From a fertilized cell of female gametophyte
  • (B) From an unfertilized cell of female gametophyte
  • (C) From isolated pollen grains
  • (D) By selective elimination of chromosomes following distant hybridization
πŸ’¬ Discuss
βœ… Correct Answer: (B) From an unfertilized cell of female gametophyte

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